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KMID : 0861420120160020162
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Korean Journal of Nuclear Medicine Technology
2012 Volume.16 No. 2 p.162 ~ p.166
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Study of the Impact of Light Through the Vitamin B12/Folate
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Cho Eun-Bit
Back Song-Ran
Kim Oi-Jung
Kim Sung-Ho
Yoon Seon-Hee
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Abstract
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Purpose:Vitamin B12 and Folate are for anemia work-up which is well known for its sensitivity of light; the
screening manual also specifies to be careful with light conditions. According to this, our laboratory minimized
the exposure of light when inspecting the Vitamin B12 and Folate, but the exposure cannot be wholly blocked due
to other various factors such as when conducting specimen segregation. Thus, this inspection is to identify to
what extent light can influence and whether the exclusion of light is mandatory during the Vitamin B12/Folate
test.
Materials and Methods:We have conducted two experiments of identifying the extent of light’s influence
when conducting the Vitamin B12/Folate test and also when specimens are under preservation. These
experiments were progressed with various concentrations of patients’ specimens which were requested to our
hospital in March 2012. The first experiment is to verify the results on Vitamin B12/Folate dependent on light
exposure during the experiment. In the process, we have compared the results of light exposure/exclusion during
the incubation process after the reagent division. The second experiment is about the impact of light exposure on
the results on Vitamin B12/Folate during the preservation. For 1, 2, 7 days the light on the specimen were wholly
blocked and were preserved under -15℃ temperature refrigeration. Then, we compared the results of
light-excluded specimen and the exposed one.
Results:When conducting first experiment, there were no
noticeable changes in the Standard and specimen’s cpm, but for Vitamin B12, the average result of specimen
exposed to light increased 7.8% compare to that of excluded one’s. Furthermore, in the significant level 0.05, the
significance probability or the p-value was 0.251 which means it has no impact. For Folate, the result being
exposed to light decreased 5.4%, the significance probability was 0.033 which means it has little impact. For the
second preservation, the result was dependent on the light exposure. The first day of preservation of Vitamin B12,
the clinical material exposed to light was 11.6%, second day clinical material exposed to light was 10.8%,
seventh day clinical material exposed to light increased 3.8%, the significance probability of the 1st, 2nd, 7th day
is 0.372, 0.033, 0.144 respectively, and which indicates that the 1st and 7th day seems to have no impact. For
Folate’s case, the clinical material exposed to light has increased 1.4% but hardly had impact, 2nd day clinical
material being exposed to light was 6.1%, 7Th day clinical material being exposed to light decreased 5.2%. The
significance probability of Folate on the 1st, 2nd, 7th day is 0.378, 0.037, 0.217 respectively, and the 1st day and the
7th day seems to have no impact.
Conclusion:After scrutinizing the impact of light exposure/exclusion, Vitamin
B12 has no impact, while Folate seems to have no noticeable influence but light exclusion is recommended due
to its significance probability of 0.033 when conducting experiment. During the preservation, the 2nd day result
depend on the light exclusion seems to have impact or influence. However, to consider the complication of the
experimental process, the experiment including technical errors is predictable. Hence, it is likely to have no
impact of light. Nevertheless, it is recommendable to exclude the light during the long preservation as per the
significance probability (p-value) of 1st and 7th day has been diminished.
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KEYWORD
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Vitamin B12 , Folate , Light exposure and exclusion , Radioimmunoassay
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